This part is still WAY under construction. However, in case anybody cares, here's what I am billing as the 'Conformational Conundrum': the emerging picture of how cs mutants we have isolated are turning out to suppress one another, and how a collection of suppressors interact with each of the cs (and other 'archetype') mutants. The 'big picture' looks like this (the key is below).

Key features are now being 'enlivened'--if you click on a given

The primary difference between the above the previous version (accessible via this LINK) is thatI have joined G680V with {L596S,P & L222F} and joined {T189I & V192F} with {N483S & Y494K & W501L}. Also, the newly discovered suppression of Y494K by G691T is indicated, and I have started distinguishing between mutants that have strong biological phenotypes on their own (written in black) and those that do not (written in white or outline font). Enjoy!

Symbols: Gray circles represent archetypes, which are currently simply groups of mutants that I have lumped together based on any of the following criteria:

• Similar biochemical properties
• Ability to suppress common target (e.g. both T189I and V192F suppress G680V)
• Ability to be suppressed by a series of mutants (e.g. N483S, Y494K and W501L can all be suppressed by E150K, M486I, P650T, I656F
• I don't know where else to put them

My dream is to one day merge some groups as needed and to correlate different genetic groupings with structural and biochemical states of the motor

Gray arrows indicate suppression, i.e. L596S and L222F can suppress (and are suppressed by) delArink, E531Q and P536R.

TRIANGLES indicate residues that suppress different gray circles depending on whether they mutate to LARGER residues (broad end of triangle; green arrows) or smaller residues (narrow end of the circle; orange arrows).

The ELLIPSE indicates residues that, when changed toward MORE POSITIVE CHARGE (blue arrows), suppress different gray circle than when they mutate to negative charge (red arrows).

Cyan rectangles indicate suppressors that act on more than one gray circle.

The black 'anti' oval containing W501R indicates that it is the 'evil twin' of W501L--it occurs at the some amino acid, but conveys opposing properties.

Caveats: Not all data has been confirmed, therefore subject to revision!
Clearly, the A574 changes don't 'make sense' when interpreted in terms of SIZE change; another property may be at work here; one intriguing possibility is a-helical propensity.

Bruce Patterson http://research.biology.arizona.edu/myosin