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P536R[G13]
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Suppressed By L222F AND 2 mutants not in myosin...actin? * = suppression is reciprocal |
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WHERE: P536R actually lives on the gridline separating F from G. It is the bend in the protein chain where the yellow changes to green (hidden from view). WHAT: Proposed by several groups to make direct interaction with actin. Giese and Spudich (Biochemistry 36:8465)) report that this mutation interferes with actin-activation, slows actin movement by wild-type myosin, and has a somewhat lower affinity for actin. P536R was one of the original cold-sensitive mutant isolates. FRIENDS & RELATIONS: By suppression criteria, it behaves similarly to the other "Actin binding" mutants E531Q[G12] and R562H[G10]. Biochemically, however, it appears to have significant differences from them. Shares several suppressors with delWrinkle as well. Some suppressors don't appear to be in the myosin gene; one cool possibility is that they are in actin! Image: P536R can be found in the ActBind Mirror
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Bruce Patterson http://research.biology.arizona.edu/myosin |
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