M486I[G7]

Suppresses

L453F [E7]
E531Q[G12]
P536R[G13]
R562H[G12]
delWrinkle[H10-11]

WHERE: M486 is located in G7 and just precedes the kink in the Camshaft (red alpha helix).

WHAT: A very interesting position and change. The Camshaft is bent in Ivan Rayment's vanadate (most common one on these pages and seen in the gridded picture) and AlF structures of truncated Dictyostelium myosin. An alteration from Met to Ile might make this bending more probable; hence M486I would favor a structure like that above over the BeF or chicken structures (in which the Camshaft is straight). An exciting 'cross-over' suppressor that can suppress actin binding site mutations as well as L453F, a member of the Cluster of mutations that can suppress G680V[G7].

FRIENDS & RELATIONS: It is one of the 3 mutations known to be capable of suppressing delWrinkle. It can also suppress the Big Three actin binding region mutants (E531Q, P536R, R562H), which gives it tentative membership in the large family of suppressors that can suppress these three mutations.

Analysis: We are currently determining which residues can substitute for methionine (M) to give rise to suppression. Currently, we have discovered that alanine and glycine can do so and are investigating proline (P)

Image: M486I can be found in the ActBind and CamShaft mirrors