L453F[E7]
Suppressed By
N38Y
F85L,S
L94F
E150K[F3]
M486I [G7]
E560K[G10]
L676I
G680V[G7]
IR685NS
R686C
* = suppression is reciprocal
WHERE: L453F is located in E7 on the coral beta strand. It is closely juxtaposed with other members of the Cluster, a group of 3-dimensionally related residues that can mutate to suppress the G680V[G7] change. Along with L176, the sidechain of L453 closely approaches the CamShaft at residue L478.
WHAT: L453F confers poor growth on Dictyostelium cells growing on bacterial lawns. The primary biochemical defect is high basal ATPase, an indication that the motor 'thinks' that it has bound actin. The direct mechanism would be through making opening of the gateway more facile; I believe that this occurs by forcing the Camshaft toward a 'gate open' configuration.
FRIENDS & RELATIONS: Shares both location and biochemical properties with other members of the Cluster, L175F, L176F, T189I[E7], V192[D6]. Through mutual suppression by M486I [G7] it is genetically linked with actin binding site mutations delWrinkle[H10-11], E531Q[G12] , P536R[G13] and R562H[G12]. Along similar lines, its E560K suppressor is suggestively similar to E560R which suppresses R562H[G12]. Through mutual suppression by E150K[F3] it is genetically linked to Y494K[I5], W501L[I5] and G691C.
Image: L453F can be found in the G680V + Supps mirror.
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