Making Myosin Mutants: an Approach to Understanding the Little Bitty Motor that moves you
Note: the beef is toward the bottom of ithis page under 'solving the cycle'-it's a compendium of all our mutant-interaction data. As is explained in the earlier sections, these represent pieces of a puzzle whose solution will be a 'movie' of the myosin motor BEING a motor...
I've tried to make this page accessible to pretty much anybody who
wants to think or learn about the problem we work on; this may alternately
frustrate experts and fail to satisfy newcomers. Your comments can help
me. Each page has a color theme; to help you remember where info is.
There's a navigation table at the bottom of most pages with likely places
to go next; the Monster Truck will always bring you Home (this page).
In the list below, sections with icons take you to specific info while
those that are text-only lead to another page of descriptions and choices.
Text in green gives a more technical description
Is there really any hope of getting clues about how a tiny machine works
by breaking it? And what hope is there of trying to fix it by
breaking it some more? For the philosophy behind our approach of mutant
making and suppressor isolation as an approach to enlightenment, delve
into this page. Rationale.
The Y2K direction of this work is an initiative to boldly go where no genetics has gone before: Deducing and ordering the shapes of the motor through its cycle. This work builds on all our findings to date, emerging hypotheses and accumulated wisdom (such as it is!). Follow the link for the rationale, the approach, and the emerging picture of an orderly, well-behaved Myosin! (Currently under construction, but there's enough there to sink your teeth into). Being expanded and updated as time and new data permit! Summary of mutants isolated, genetic interactions observed; functional relationships between mutants
Hot new stuff! Added our latest genetic and intellectual
data concerning three highly conserved residues that form a cluster in most
crystal structures and which each showed up as cold-sensitive mutants in
our initial genetic screen. Follow this
link to get right to the meat of the problem (or go straight to our
Ye Olde Stuffe: Pages that were once an active
part of the site, but have been 'retired'. Kept just in case anybody used